Journal: Journal of Virology
Article Title: Expression of Varicella-Zoster Virus Immediate-Early Regulatory Protein IE63 in Neurons of Latently Infected Human Sensory Ganglia ▿
doi: 10.1128/JVI.02416-09
Figure Lengend Snippet: Demonstration of neuromelanin. TGG sections from subject V (A and C) and DRG sections from subject M (B and D) are shown. Panels A and B are stained with Nile blue solution, which differentiates neuromelanin (long arrow and green staining) and lipofuscin (short arrow and blue staining). Panels C and D are stained with Nile blue solution after acetone extraction, which removes lipofuscin. Arrows show green-stained melanins which resist acetone extraction. Panels E and F are adjacent DRG sections from subject M that were stained with Azure B (E) or antibody to synaptophysin (NBT-BCIP; 4-min development). The short arrows in panels E and F indicate neuromelanin. Magnification for all images, ×200.
Article Snippet: The reaction product was visualized using the NBT-BCIP detection kit (Vector Laboratories).
Techniques: Staining